FAQ

Although GlutenTox Home makes testing for gluten a simple process, the science behind gluten detection is actually quite complicated and the technology in the test is the result of many years of dedicated research. If you have questions about the GlutenTox Home gluten test kit, you are in the right place!

We want you to be comfortable and confident when you’re testing, so we’ve compiled a list of frequently asked questions. If yours isn’t here, please let us know.

• What is GlutenTox Home?
• Why does the GlutenTox Home gluten test kit work?
• How sensitive is GlutenTox Home?

• How should I dispose of the kit after use?
• At what temperature should I store the kit?
• What is the expiration date of the kit?
• Who makes/distributes GlutenTox Home?

• How do I test a food for gluten?
• Does the test recognize oat?
• Why is the kit not recommended for chocolate?
• Are there other items GlutenTox Home doesn’t work with?

• How can I modify GlutenTox Home to test surfaces?
• I want to test a vitamin or supplement. Can I?
• I want to test a gelcap. Can I?

• When I applied the dilution solution onto the test stick, I saw a pink flush go across the test window. Is this normal?
• After 10 minutes, the test showed a negative result (only the blue control line appeared in the window). But when I checked the test stick the next day, I noticed a faint pink line. What’s going on?
• I got a test result that surprises me. What do I do?

• What is difference between the G12 antibody used in GlutenTox and other antibodies?
• What happens if the toxic fragment of the gluten molecule is cleaved during hydrolyzation?
• What kind of safety qualifications does GlutenTox Have?
• Where can I read more about the science behind GlutenTox and the G12 antibody?

• Can I use GlutenTox Home for my Science Fair project?
• I see that a mortar & pestle and a timer are helpful for testing. Do you sell them?
• What if I have more questions about the GlutenTox Home gluten test kit?
• I’d like more specific information; I’m a:

  • doctor, dietitian, nutritionist or other health professional.
  • leader for my local gluten-free support group.
  • retailer interested in stocking GlutenTox Home.
  • manufacturer making tasty, gluten-free food.
  • commercial kitchen serving some gluten-free customers.

What is GlutenTox Home?

A quick, user-friendly test for the detection of gluten in foods and liquids. More specifically: a lateral flow device that uses the G12 antibody to detect the specific fraction of gluten that’s harmful for people with celiac disease. ^

Why does the GlutenTox Home gluten test kit work?

The G12 antibody, located inside the test stick, is specially designed to seek out the 33-mer peptide of the alpha-gliadin molecule. Studies show that this peptide is the primary cause of the auto-immune response in celiac patients. Accordingly, a food will test positive with GlutenTox Home if it contains these toxic peptides. G12 is able to recognize gluten in wheat, barley, rye and oat (for more on oat toxicity, see below). It is also uniquely well-suited to detecting gluten in hydrolyzed, fermented, or otherwise processed foods. ^

How sensitive is GlutenTox Home?

You can choose the sensitivity of each test you perform: either 20ppm or 5ppm. According to the Codex Alimentarius Commission, food can be considered “gluten-free” if the amount of gluten does not exceed 20 parts per million (ppm), and is considered as “very low in gluten content” if the amount of gluten does not exceed 100 ppm. The FDA’s proposed legislation regarding gluten-free labeling also indicates 20ppm as the threshold underneath which food can be considered gluten-free. However, we know that many people are particularly sensitive and many commercial kitchens strive to guarantee an additional level of safety for their customers. Because of this, instructions to test to 5ppm are included with the kit. ^

How should I dispose of the kit after use?

All components of the kit are non-toxic and therefore fully disposable in ordinary trash. They can be recycled where appropriate according to the material. ^

At what temperature should I store the kit?

The kit can be stored at 35°F – 85°F. ^

What is the expiration date of the kit?

A conservative expiration date is indicated on each foil pouch. Other materials (extraction solution, dilution solution) do not expire. You will know if you are using a test that has expired because the blue control line will not appear. ^

Who makes/distributes GlutenTox Home?

All GlutenTox kits are made by Biomedal Diagnostics, in sunny Seville, Spain. Biomedal is a fully accredited facility, holding ISO certifications for the production of gluten test kits as well as on-site gluten analysis.

Emport, LLC distributes GlutenTox in North America. Emport has one simple goal: more safe food for more happy people. The company is based in cloudy Pittsburgh, PA and headed by Emily Kaufman, who’s been gluten-free since 2002. ^

How do I test a food for gluten?

Here’s a video that shows just how easy it is to use GlutenTox (click here for instructions in Spanish ^).

The sample should be at room temperature, and most importantly, never hot.

Does the test recognize oat?

Yes. The test can detect both cross-contamination in oats, and also those strains of oat that contain gluten. A positive signal will appear if the oat being tested is potentially harmful for celiac disease patients. If you’d like to read more about gluten in oats, we recommend this study on gluten in oats or visit Celiac.com for an analysis of the study. ^

Why is the kit not recommended for chocolate?

Gluten from chocolate or cocoa is very hard to extract; for this reason the level of gluten can be underestimated. However, if you get a positive result with chocolate, it definitely does contain gluten and should not be consumed by anyone on a gluten-free diet. ^

Are there other items GlutenTox Home doesn’t work with?

  1. Maggi – Seasoning – Arôme (liquid sauce): This seasoning sauce contains gluten as described by the manufacturer in its ingredients list. Note that its manufacturing process and other active ingredients are not suitable for GlutenTox Home. Consider this food as containing gluten.
  2. Soy Sauce: Naturally brewed soy sauce undergoes fermentation process that often hydrolyzes/breaks down gluten to level close or below 20 ppm.
  3. Oil: Oils are inherently gluten-free but can be contaminated with crumbs of gluten-containing items. To verify whether or not a deep fryer or Fryolator is safe for gluten-free foods, it is recommended that you filter the oil and test any residues/crumbs that remain. Pat the crumbs dry as best as possible, and test them with GlutenTox according to the protocol for crumbs. If the test is negative, the oil can be considered safe.
  4. Highly processed and complex matrices, as well as those with polyphenols and/or antioxidants: Although GlutenTox tests have been successfully tested against a wide variety of highly-processed non-food items, there may be some cases in which a more thorough extraction process is required to identify traces of gluten. If you are planning to test a vitamin, supplement, medication, or products with polyphenols (chocolate, tannins, etc.) that you feel may be highly processed, please contact us with more information about the specific item. In all cases, a positive test result always indicates the presence of gluten: there are no false positives.
  5. Supplements or personal care products that are very high in silica should not be tested using GlutenTox, and often require special analysis in a laboratory setting. ^

How can I modify GlutenTox Home to test surfaces?

  1. Use a thin blade to pry open the plastic casing of the GlutenTox Home test stick.
  2. Inside is a thin test strip; remove it with clean hands and rub the cottony end (where you would normally drop the dilution solution) cotton-down on 2.5 square inches of any hard surface.
  3. Open a dilution vial; use a clean pipette to transfer 6 drops of solution into the vial’s overturned cap.
  4. Dip the cottony end of the test strip into the solution for 5 seconds until the solution is absorbed, then lay the strip somewhere flat.
  5. You can read the results in 10 minutes, as with any other GlutenTox Home test. A positive result indicates that a kilo of dough, kneaded on the surface, would contain at least 0.01 ppm gluten. ^

I want to test a vitamin or supplement. Can I?

Yes, however please note that GlutenTox Home has been designed for testing food items; there may be some vitamins or supplements with complex matrices that require additional steps in processing to fully extract the gluten. Please contact us for more information.

In all cases, a positive result indicates the presence of gluten above the designated threshold. Please be sure to adequately grind the sample prior to testing, and to use the full gram / two level spoonfuls for your test. ^

I want to test a gelcap. Can I?

Yes, however please note that there may be some medications or supplements with complex matrices that require additional processing to fully extract the gluten (a positive result always does indicate the presence of gluten). When testing a gelcap, to get the most accurate results possible, we recommend modifying the protocol slightly:

  • Test the contents and the capsule separately (this requires two tests).
  • Test the contents as you would test any other solid sample.
  • Because the gel capsules are quite hard, we recommend these additional steps:
    • Cut the capsules into the smallest pieces possible, using a sharp knife or clean scissors
    • Soak the pieces of capsule in the extraction solution for at least ten minutes before proceeding with the test.
    • Vigorously shake the sample in the extraction solution for a full five minutes, intead of the normal two.
    • Complete the test according to normal instructions. ^

When I dropped the dilution solution onto the test stick, I saw a pink flush go across the test window. Is this normal?

Yes. You’ll notice that the pink dye in the kit travels across the window as the dilution solution is absorbed by the test. This does not indicate the presence of gluten in the sample! If there is gluten in the sample, a pink line will appear in the window within 10 minutes of using the test. ^

After 10 minutes, the test showed a negative result (only the blue control line appeared in the window). But when I checked the test stick the next day, I noticed a faint pink line. What’s going on?

The test results should be read at 10 minutes. Any faint lines that appear after this time limit are not valid and do not indicate the presence of gluten in the sample. Conversely, positive results may fade in intensity after 10 minutes. If the test shows a pink line and a blue line at 10 minutes, it’s positive. If the test shows only a blue line at 10 minutes, it’s negative. ^

I got a test result that surprises me. What do I do?

If your results are unexpected, please email us with more information about your test. We’ll work with you until your questions are resolved. ^

What is difference between the G12 antibody used in GlutenTox and other antibodies?

The G12 antibody specifically recognizes the most toxic fragment of the gliadin present in gluten, called 33-mer. Because gliadin is often hydrolyzed in foods and drinks, less-specific antibodies may underestimate the amount of net toxic fractions. In addition, the G12 antibody has never shown any cross-reactivity with soy or other gluten-free foods. In all cases, the G12 antibody will always be able to detect if a sample is toxic for celiac patients because it specifically detects that toxic fraction; it is therefore much more reliable than classic antibodies for hydrolyzed food such as cookies, beer, baby food, etc. ^

What happens if the toxic fragment of the gluten molecule is cleaved during the hydrolyzation process?

Being cleaved, the fragment will not be detected by the G12 antibody — BUT it will also lose its toxicity. This sample is therefore suited for celiac patients (Morón et al. 2008). ^

What kind of safety qualifications does GlutenTox Have?

Biomedal Diagnostics has numerous positive evaluations for its gluten test kits and in-house gluten testing: AESAN (The Spanish Agency for Food Safety and Nutrition) has positively evaluated the GlutenTox Lateral Flow Device as an accurate method for gluten detection in any types of food. AESAN is an organization attached to the Spanish Ministry of Heath and Social Politics, whose role is to ensure the highest standards for safety and promote citizens health. The test kits have also passed the FAPAS proficiency tests, an international standard. ^

Where can I read more about the science behind GlutenTox and the G12 antibody?

Please visit our Links and Press page for a list of relevant studies. ^

Can I use GlutenTox Home for my Science Fair project?

Yes! GlutenTox can be a great way to explain lateral flow / immunochromatographic devices, to show the prevalence of gluten in non-certified foods, etc. However, there’s one very important thing we want to remind you of: test results will fade over time. If you are planning to display positive test results, you’ll need to perform the test right before exhibiting to ensure it is as bright as possible. Alternately, we suggest taking photographs to use in your project.

If you’d like to discuss your planned experiment with us before using the test kits, please do! We always love hearing about science fair projects and we might even have some ideas to help make your presentation even more complete. ^

I see that a mortar & pestle, scale and timer are helpful for testing for gluten. Do you sell them?

We do not currently sell these items. However, all of the items are available via Amazon.com, as well as many other fine online retailers.
^

What if I have more questions about the GlutenTox Home gluten test kit?

Send us a note or give us a call! We welcome any and all questions, any time. ^

I’d like more specific information…

We have special programs for health professionals and retailers, and additional products specially designed for larger kitchens. Check out our Commercial / Wholesale page for more information! ^